Source folder: Q&As
Source workbook: Questions and answers.xlsx
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Questions and answers imported from the source spreadsheet.
Question: Why do we need to redraw
Answer:
We have a certain metric that we must hit to report the case and if it comes back High Ambient there is something in the mother’s plasma not allowing us to do that. That does not mean there is something wrong or anything there was just not sufficient DNA to complete the test. I would not give all that information to the patient though.
Question: Can we test when it is IVF
Answer:
We can do NIPPT for IVF but need to know if it is singleton or twin pregnancy. This is with DNAQ
Question: Can we test if twins
Answer:
Yes - If it is Twins, all alleged father samples must be provided. This is with DNAQ
Question: If the mother has had a blood transfusuion
Answer:
We can accept samples from patients who have received blood transfusions more than 1 year ago. If she does go ahead with testing we request that she provide the approximate date of transfusion (month & year will suffice). This is with DNAQ
Question: re-collection due to Low fetal DNA concentration
Answer:
The concentration of fetal DNA in maternal blood increases with gestational age and it varies in different people. Biological factors including a high BMI and poor trophoblast implantation are associated with a low fetal fraction. There is also variation in how quickly any given fetus will grow, just as there is variation in how quickly children and adults may grow and develop throughout their lives. As a pregnancy progresses and the fetus grows larger, the amount of circulating fetal DNA will naturally increase. A re-collection at a later gestational age will often obtain a results. This is why optimal testing is from 10 weeks gestational age, however some cases may still be low at 10+ weeks.
Question: can the result be 99.999 percent for the brother ? or the alleged father will result as 99.999 and if it's the brother will the results come out as 98 or 97% and that way she can distinguish if its the brother or the alleged father.
Answer:
Close blood relatives cannot be distinguished between, the only was to have a definitive answer is if one is excluded.
We must also acknowledge the possibility that both alleged father’s may match as they are genetically similar.
Question: Haemolysis & Sample Degradation
Answer:
Depending on collection and transport of the sample there is a possibility that the sample will be compromised due to haemolysis or degradation. Haemolysis is damage to red blood cells and can be caused by a difficult bleed/collection, inadequate mixing of the blood sample with the preservative in the tube, delayed transport or extreme temperatures.
Question: Blood Transfusion
Answer:
We can accept samples from patients who have received blood transfusions more than 1 year ago.
If she does go ahead with testing we request that she provide the approximate date of transfusion (month & year will suffice).
Question: Mother had a miscarriage one week before getting pregnant
Answer:
Yes, that is fine as long as she is at least 7 weeks along that is not a problem and as long as Father A & Father B are not related this can be done.
Question: Surrogacy Cases
Answer:
Our NIPP test is not validate for surrogacy cases, therefore, this test wouldn't have been analyzed had the lab been informed for this situation. Please note that the following information is part of the statement provided on the NIPP reports as of June of 2019. This was changed at the end of June in conjunction with the AABB accreditation for NIPP tests for our chain cases. All corporate accounts should have been informed at this time. The major change was we added an AF confirmation sample to the test. We changed the verbiage on all tests to be consistent with AABB requirements. We now provide the actual number of SNPs, that are used in the algorithm, a statement indicating that we are using an algorithm and not the standard paternity calculations. The removal of race as it is not part of the algorithm calculation but uses a race neutral database and the statements to indicate under what conditions this test is not applicable ( related fathers, incest, in vitro/egg donor and bone marrow transplant/chimerism.)